Journal: Experimental Biology and Medicine

Article Title: Nuclear alpha spectrin: Critical roles in DNA interstrand cross-link repair and genomic stability

doi: 10.1177/1535370216662714

Figure Lengend Snippet: αIISp and XPF co-localize to nuclear foci after treatment of normal cells with a DNA ICL agent. Normal human lymphoblastoid cells were either undamaged or treated with 8-MOP plus UVA light and the localization of αIISp and XPF foci in the nucleus examined 15 h after treatment. Dual staining was carried out using a monoclonal anti-α-spectrin antibody and a polyclonal anti-XPF antibody. When fluorescent signals for the αIISp and XPF were merged, the overlapping foci were yellow, indicating co-localization of these two proteins. Cells were also stained with the appropriate preimmune sera. (Reproduced from Sridharan et al.17 with permission from the Company of Biologists Ltd.)

Article Snippet: Cells were also stained with the appropriate preimmune sera. (Reproduced from Sridharan et al. 17 with permission from the Company of Biologists Ltd.) αIISp is needed in recruitment of XPF to sites of ICLS αIISp plays a critical role in the recruitment of XPF to sites of ICLs during the repair process as is demonstrated by studies which show that, after knockdown of αIISp, XPF fails to localize to these sites of damage.

Techniques: Staining